Features and Benefits

Compatible with high-throughput handling systems.

General description

Oxalate Decarboxylase (OXDC, EC 4.1.1.2) catalyzes the conversion of oxalate to formate and carbon dioxide in the presence of molecular oxygen. OXDC-responsive proteins are involved in the regulation of metabolism and stress responses in plants.

Principle

The Oxalate Decarboxylase Activity Assay Kit provides a simple and high throughput adaptable procedure for measuring OXDC activity in a variety of plant and fungal tissues. OXDC activity is determined by generating a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of oxalate decarboxylase is the amount of enzyme required to generate 1.0 µmole of formate per minute at pH 5 at 37 °C.

Suitability

Suitable for the detection of Oxalate Decarboxylase activity in samples such as plants and fungi.